Preparation and biodistribution study of 67Ga-gallium calcitonin

نویسندگان

  • Amir Reza Jalilian Radiopharmaceutical Research and Development Lab, Nuclear Science and Technology Research Institute, Atomic Energy Organization of Iran, Tehran, Iran
  • Fatemeh Bolourinovin Radiopharmaceutical Research and Development Lab, Nuclear Science and Technology Research Institute, Atomic Energy Organization of Iran, Tehran, Iran
  • Mohammad Amin Hosseini Radiopharmaceutical Research and Development Lab, Nuclear Science and Technology Research Institute, Atomic Energy Organization of Iran, Tehran, Iran
چکیده مقاله:

Introduction: In order to develop a radiolabeled calcitonin (CT) derivative for receptor imaging studies, CT was successively labeled with 67Ga-gallium chloride. Methods: The best results of the conjugation were obtained by the addition of 0.5 ml of a CT nasal pharmaceutical solution (1100 IU) to a glass tube pre-coated with DTPA-dianhydride (0.01 mg) at 25°C with continuous mild stirring for 30 min. Results: After solid phase purification of the radiolabeled hormone, instant thin layer chromatography (ITLC) showed radiochemical purity of higher than 95 % at optimized conditions (specific activity =67-134 KBq/IU, labeling efficiency 70%). 67Ga-DTPA-CT mainly accumulates in the liver. Conclusion:  Preliminary in vivo studies (ID/g%) in male wild-type rats showed significant liver uptake of the tracer after 24 hours. 67Ga-DTPA-CT can be a suitable probe for biodistribution study of CT receptors in various physiological as well as neoplastic lesions with over-expressed calcitonin receptors.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

preparation and biodistribution study of 67ga-gallium calcitonin

introduction: in order to develop a radiolabeled calcitonin (ct) derivative for receptor imaging studies, ct was successively labeled with 67ga-gallium chloride. methods: the best results of the conjugation were obtained by the addition of 0.5 ml of a ct nasal pharmaceutical solution (1100 iu) to a glass tube pre-coated with dtpa-dianhydride (0.01 mg) at 25°c with continuous mild stirring for 3...

متن کامل

Preparation and biodistribution study of Ga-gallium calcitonin

Introduction: In order to develop a radiolabeled calcitonin (CT) derivative for receptor imaging studies, CT was successively labeled with Ga-gallium chloride. Methods: The best results of the conjugation were obtained by the addition of 0.5 ml of a CT nasal pharmaceutical solution (1100 IU) to a glass tube pre-coated with DTPA-dianhydride (0.01 mg) at 25C with continuous mild stirring for 30 ...

متن کامل

Preparation and Biodistribution of [67Ga]-labeled- oxytocin for SPECT purposes

Background: Oxytocin (OT) is a paracrine hormone with various biological activities and many sex organs in both sexes, as well as many tumor cells have shown to have related receptors. In this study the development of a receptor imaging tracer for possible tumor imaging has been described. Materials and Methods: OT was successively labeled with [67Ga]-gallium chloride after conjugation with fre...

متن کامل

preparation and biodistribution of [67ga]-labeled- oxytocin for spect purposes

background: oxytocin (ot) is a paracrine hormone with various biological activities and many sex organs in both sexes, as well as many tumor cells have shown to have related receptors. in this study the development of a receptor imaging tracer for possible tumor imaging has been described. materials and methods: ot was successively labeled with [67ga]-gallium chloride after conjugation with fre...

متن کامل

Preliminary Dosimetry Study of 67Ga-AATS for Human Based on Biodistribution Data in Rats

Introduction Gallium-67 (67Ga) has been used as a radionuclide for imaging a variety of solid tumors since 1969. Since then use of various gallium-based radiotracers has been reported. Recently, 67Ga-labeled acetylacetate bis(thiosemicarbazones) (67Ga-AATS) complex with significant tumor accumulation and fast blood clearance has been employed. Materials and Methods In this study, the absorbed d...

متن کامل

Preparation and biodistribution study of a 99mTc-labeled toxic fraction of Iranian mesobuthus eupeus scorpion venom

  Introduction: Iranian scorpion species are classified in Buthidae and Scorpionidae with 16 genera and 25 species. In Iran, similar to other parts of the world, there are a few known species of scorpions responsible for severe envenoming. Mesobuthus eupeus is the most common species in Iran. Its venom contains several toxin fractions which can affect the ion...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 19  شماره 2

صفحات  38- 45

تاریخ انتشار 2011-12-01

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023